Recent Publications

• K Mizushima, Y Kumamoto, S Tamura, M Yamanaka, K Mochizuki, M Li, S Egoshi, K Dodo, Y Harada, NI Smith, M Sodeoka, H Tanaka, K Fujita, "Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging", Science Advances , in press (2024).
• AJ Hobro, T. Sakaguchi, S. Akira, NI Smith, "Correlative quantitative Raman chemical imaging and MCR-ALS in mouse NASH model reveals direct relationships between diet and resultant liver pathology", Chemical & Biomedical Imaging 2 (8), pp. 577–583 (2024).
• N. Pavillon, E. L. Lim, A. Tanaka, S. Hori, S. Sakaguchi, N. I. Smith, "Non-invasive detection of regulatory T cells with Raman spectroscopy", Scientific Reports 14 (1), pp. 14025 (2024).
• AJ Hobro, N. Pavillon, K. Koike, T. Sugiyama, T. Umakoshi, P. Verma, K. Fujita, NI Smith, "Imaging vs non-imaging Raman spectroscopy for high-throughput single-cell phenotyping", Analytical Chemistry 96 (18), pp. 7047-7055 (2024).
• AJ Hobro, NI Smith, "Spontaneous Raman bioimaging – Looking to 2050", Vibrational Spectroscopy 131 (1), pp. 103668 (2024).

2023

• P. M. Lelliott, A. J. Hobro, N. Pavillon, M. Nishide, Y. Okita, Y. Mizuno, S. Obata, S. Nameki, H. Yoshimura, A. Kumanogoh, N. I. Smith, "Single-cell Raman microscopy with machine learning highlights distinct biochemical features of neutrophil extracellular traps and necrosis", Scientific Reports 13 (1), pp. 10093 (2023).
• K Mochizuki, Y Kumamoto, S Maeda, M Tanuma, A Kasai, M Takemura, Y Harada, H Hashimoto, H Tanaka, NI Smith, K Fujita, "High-throughput line-illumination Raman microscopy with multislit detection", Biomedical Optics Express 14 (3), pp. 1015-1026 (2023).
• N Pavillon, NI Smith, "Non-invasive monitoring of T cell differentiation through Raman spectroscopy", Scientific Reports 13 (1), pp. 3129 (2023).

• K Bando, S Yabuuchi, M Li, T Kubo, R Oketani, NI Smith, K Fujita, "Bessel-beam illumination Raman microscopy", Biomedical Optics Express 13 (6), pp. 3161-3170 (2022).
• K Temma, R Oketani, R Lachmann, T Kubo, NI Smith, R Heintzmann, K. Fujita, "Saturated-excitation image scanning microscopy", Optics Express 30 (8), pp. 13825-13838 (2022).
• PM Lelliott, M Nishide, N Pavillon, Y Okita, T Shibahara, Y Mizuno, H. Yoshimura, S. Obata, A. Kumanogoh and N. I. Smith,"Cellular Adhesion Is a Controlling Factor in Neutrophil Extracellular Trap Formation Induced by Anti-Neutrophil Cytoplasmic Antibodies", ImmunoHorizons 6 (2), pp. 170-183 (2022).
• K. Koike, N. Smith, K. Fujita ,"Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy", Biomedical Optics Express 13 (2), pp. 995-1004 (2021).

2021

• T. Kubo, K. Temma, K. Sugiura, H. Shinoda, K. Lu, N. Smith, T. Matsuda, T. Nagai, K. Fujita ,"Visible-wavelength multiphoton activation confocal microscopy", ACS Photonics 8 (9), pp. 2666-2673 (2021).
• N. Pavillon, and N.I. Smith, "Deriving accurate molecular indicators of protein synthesis through Raman-based sparse classification", Analyst 146, pp. 3633-3641 (2021).
• F. Tai, K. Koike, H. Kawagoe, J. Ando, Y. Kumamoto, N.I. Smith, M. Sodeoka, and K. Fujita "Detecting nitrile-containing small molecules by infrared photothermal microscopy", Analyst 146, pp. 2307-2312 (2021).

• K. Koike, K. Bando, J. Ando, H. Yamakoshi, N. Terayama, K. Dodo, N.I. Smith, M. Sodeoka, and K. Fujita, "Quantitative Drug Dynamics Visualized by Alkyne-Tagged Plasmonic-Enhanced Raman Microscopy", ACS Nano 14 (11), pp.15032-15041 (2020).
• T. Kubo, K. Temma, N.I. Smith, K. Lu, T. Matsuda, T. Nagai, and K. Fujita, "Hyperspectral two-photon excitation microscopy using visible wavelength", Optics Letters 46 (1), pp.37-40 (2020).
• T. Sugiyama, A. J. Hobro, N. Pavillon, T. Umakoshi, P. Verma, and N. Smith, "Label-free Raman mapping of saturated and unsaturated fatty acid uptake, storage, and return toward baseline levels in macrophages", Analyst 146 (4), pp. 1268-1280 (2020).
• K. Nishida, G. Deka, N. I. Smith, S. W. Chu, and K. Fujita, "Nonlinear scattering of near-infrared light for imaging plasmonic nanoparticles in deep tissue", ACS Photonics 7 (8), pp. 2139-2146 (2020).
• P. M. Lelliott, M. Momota, T. Shibahara, M. S. J. Lee, N. I. Smith, K. J. Ishii, and C. Coban, "Heparin induces neutrophil elastase-dependent vital and lytic NET formation", Int. Immunol. 32 (5), pp. 359-368 (2020).

• N. Pavillon and N. I. Smith, "Immune cell type, cell activation, and single cell heterogeneity revealed by label-free optical methods", Sci. Rep. 9, p. 17054 (2019).
• R. Oketani, H. Suda, K. Uegaki, T. Kubo, T. Matsuda, M. Yamanaka, Y. Arai, N. I. Smith, T. Nagai and K. Fujita, "Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging, J. Biomed. Opt. 25(1), p. 014502 (2019).

• Y. Nawa, Y. Yonemaru, A. Kasai, R. Oketani, H. Hashimoto, N. I. Smith and K. Fujita, "Saturated excitation microscopy using differential excitation for efficient detection of nonlinear fluorescence signals", APL Photonics 3(8), p. 080805 (2018).
• N. Pavillon, A. J. Hobro, S. Akira and N. I. Smith, "Noninvasive detection of macrophage activation with single-cell resolution through machine learning", Proc. Natl. Acad. Sci. USA 115(12), pp. E2676-E2685 (2018).
• A. J. Hobro and N. I. Smith, "Vibrational spectroscopic imaging of pathogens, microorganisms, and their interactions with host systems", Opt. Commun. 422, pp. 75-84 (2018).

• A. J. Hobro and N. I. Smith, "An evaluation of fixation methods: spatial and compositional cellular changes observed by Raman imaging", Vib. Spectrosc. 91, pp. 31-45 (2017).
• R. Oketani, A. Doi, N. I. Smith, Y. Nawa, S. Kawata and K. Fujita, "Saturated two-photon excitation fluorescence microscopy with core-ring illumination", Opt. Lett. 42(3), pp. 571-574 (2017).

• N. Pavillon and N. I. Smith, "Compressed sensing laser scanning microscopy", Opt. Express 24(26), pp. 30038-300052 (2016).
• A. J. Hobro, Y. Kumagai, S. Akira and N. I. Smith, "Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination", Analyst 141, pp. 3756-3764 (2016).
• Y. Kumamoto, K. Fujita, N. I. Smith and S. Kawata, "Deep-UV biological imaging by lanthanide ion molecular protection", Biomed. Opt. Express 7(1), pp. 158-170 (2016).

• K. Watanabe, A. F. Palonpon, N. I. Smith, L.-d. Chiu, A. Kasai, H. Hashimoto, S. Kawano and K. Fujita, "Structured line illumination Raman microscopy", Nat. Commun. 6, pp. 10095 (2015).
• N. I. Smith and N. Pavillon, "Fabrication of Gold Nanoparticles inside Cells by Laser Irradiation and their Application as Surface-Enhanced Raman Probes", レーザ加工学会誌 (Japan Laser Processing Society Journal) 22(3), pp. 228-232 (2015).
• K. Bando, N. Smith, J. Ando, K. Fujita and S. Kawata, "Analysis of dynamic SERS spectra measured with a nanoparticle during intracellular transportation in 3D", J. Opt. 17(11), pp. 114023 (2015).
• M. Yamanaka, K. Saito, N. I. Smith, Y. Arai, K. Uegaki, Y. Yonemaru, K. Mochizuki, S. Kawata, T. Nagai and K. Fujita, "Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging", J. Biomed. Opt. 20(10), pp. 101202-1-101202-11 (2015).
• Y. Yonemaru, A. F. Palonpon, S. Kawano, N. I. Smith, S. Kawata and K. Fujita, "Super-Spatial- and -Spectral-Resolution in Vibrational Imaging via Saturated Coherent Anti-Stokes Raman Scattering", Phys. Rev. Appl. 4, pp. 014010 (2015).
• L.-d. Chiu, A. Palonpon, N. I. Smith, S. Kawano, M. Sodeoka and K. Fujita, "Dual-polarization Raman spectral imaging to extract overlapping molecular fingerprints of living cells", J. Biophotonics 8(7), pp. 546-554 (2015).
• N. Pavillon and N. I. Smith, "Implementation of simultaneous quantitative phase with Raman imaging", EPJ Techniques and Instrumentation 2(5), pp. 1-11 (2015).
• A. J. Hobro, N. Pavillon, K. Fujita, M. Ozkan, C. Coban and N. I. Smith, "Label-free Raman imaging of the macrophage response to the malaria pigment hemozoin", Analyst 140, pp. 2350-2359 (2015).
• N. Pavillon and N. I. Smith, "Maximizing throughput in label-free microspectroscopy with hybrid Raman imaging", J. Biomed. Opt. 20(1), pp. 016007-1-016007-10 (2015).